Effect of Photobiomodulation on Transforming Growth Factor-β1, Platelet-Derived Growth Factor-BB, and Interleukin-8 Release in Palatal Wounds After Free Gingival Graft Harvesting: A Randomized Clinical Study
Keskiner I, Lutfioglu M, Aydogdu A, Saygun NI, Serdar MA.Photomed Laser Surg. 2016;34(6):263-271
Objective
This study evaluated the impact of photobiomodulation (PBM) on the healing of the donor palatal area
following free gingival graft (FGG) harvesting by examining changes in transforming growth factor (TGF)-b1,
platelet-derived growth factor (PDGF)-BB, and interleukin (IL)-8 levels in palatal wound fluid (PWF).
Material and methods
Thirty patients were selected and randomly assigned to receive PBM (laser group) or PBM sham
(sham group) in the palatine area after FGG harvesting. A neodymium-doped yttrium aluminum garnet (Nd:YAG)
laser (1064 nm) was applied to the test sites immediately after surgery and every 24 h thereafter for 4 days. PWF
was collected on Days 7 and 12, and PWF TGF-b1, PDGF-BB, and IL-8 levels were analyzed by enzyme-linked
immunosorbent assays (ELISA).
Results
PWF TGF-b1, PDGF-BB, and IL-8 levels were significantly lower on
Day 12 than on Day 7 for both groups. PWF TGF-b1, PDGF-BB, and IL-8 levels of the laser group were
significantly higher than those of sham group on Day 7 ( p < 0.05). PWF TGF-b1 levels were also significantly
higher in laser group than in the sham group on Day 12; however, differences in PDGF-BB and IL-8 levels between
groups on Day 12 were statistically nonsignificant.
Conclusions
Observed increases in PWF TGF-b1, PDGF-BB,
and IL-8 levels suggest that PBM may accelerate wound healing by stimulating production of selected mediators.
